Expertise

Preparation and storage of samples

• Preparation and dispensing of microbiological culture media
• Sterilization of microbiological media, small glass and laboratory equipment
• Environmental samples homogenization
• Preparation of inoculating fluids and diluter used in microbiological analyses
• Preparation of microscope slides and automatically stain using Gram method
• Preparation and culturing of fungi in microcultures
• Storage of bacterial and fungal strains in microbanks in low temperature freezer (to -85 ° C)

Analyses based on molecular biology techniques

• Selection and optimization of the DNA isolation methods from environmental samples, mycelium and bacteria and yeast cells
• Identification of bacteria based on 16S rRNA sequencing analysis
• Identification of fungi based on D2 fragment sequencing, located within the gene encoding the large subunit of the ribosome (LSU)
• Analysis of genetic profile of microorganisms by amplified fragment length polymorphism (AFLP)
• Phylogenetic analysis of bacterial and fungal strains isolated from environmental samples
• Genetic diversity of bacterial and fungal communities using polymerase chain reaction and denaturing  gradient gel electrophoresis (PCR-DGGE) and terminal restriction fragment length polymorphism (t-RFLP)
• Detection of microorganisms, especially heat resistant fungi by the moloecular biology methods (PCR, qPCR, LAMP)
• Detection and expression analysis of genes encoding cellulose degradation enzymes (PCR, qPCR, qRT-PCR)

Analyses based on BIOLOG System

• Identification and characterization of metabolic profile of fungi (FF plates)
• Identification and characterization of metabolic profile of bacteria(GEN III plates)
• Identification and characterization of metabolic profile of yeast (YT plates)
• Identification and characterization of metabolic profile of anaerobic microorganisms(AN plates)
• Functional microbial diversity evaluation based on characterization of soil metabolic profile (ECO plates)
• Microorganisms analysis by phenotype microarrays method (PM plates)
• Evaluation of microorganisms sensitivity to chemical compounds, plant extracts and fungicides (MT2 plates)
  
• Assessment of various compounds, waste and products degradation by microorganisms (MT2 plates)
  

Classical microbiological methods

• Total number of bacteria and fungi evaluation in environmental samples
• Evaluation number of heat resistant fungi and their isolation from environmental samples
• Qualitative and quantitative evaluation of proteolytic, pectinolytic, cellulolytic and amylolytic, microorganisms, ammonifying and nitrifying bacteria in environmental samples
• Identification of fungi based on macro- and microscopic observations
• Soil respiratory activity evaluation
• Assess the sensitivity of fungi to fungicides
• Evaluation of the microbiological air contamination using air sampler
• Evaluation of water microbial quality using membrane filter method

Classical biochemical analyses

• Enzymatic activity evaluation of bacterial and fungal strains (cellulase, pectinesterase, polygalacturonase, protease, lipase)
• Soil enzymatic evaluation using spectrophotometric methods (dehydrogenases, protease, urease, acid and alkaline phosphatase, β-glucosidase)
• Evaluation of ammonification and nitrification rate in soil environment
• Evaluation of soil enzymatic activity using fluorimetric methods (leucine aminopeptidase, esterase, acid and alkaline phosphatase, arylosulphatase, β-glucosidase)