Falling number method and amylograph test are methods widely used to determine the alpha-amylase activity of wheat flour. By comparison, mixolab might be used to measure dough characteristics such as protein quality, starch gelatinization, enzymatic activity and starch retrogradation in a single test. The objective of this study was to determine the variability of, and relationship among falling number, amylograph properties and mixolab parameters which characterised starch complex. Test was performed on 76 samples of wheat flour type 550 and type 750 produced in Poland. Mixolab parameters which characterise starch gelatinisation (C3), amylolytic activity (C4) and starch retrogradation (C5) allowed to better differentiate types of flour than falling number method or amylograph test. Falling number, maximum viscosity and final temperature of gelatinisation were positively correlated with starch gelatinisation (C3), amylolytic activity (C4, difference of the points C3C4) and starch retrogradation (C5, difference of the points C5C4).