The aim of the research was to analyse the impact of soil water potential on the concentration of aluminium and selected mineral nutrients in soil solution.Soil acidification is a natural process accelerated by agriculture, and one of the most important factors limiting crop production worldwide. Concentrations of aluminium and selected mineral nutrients in solutions obtained from soil at initial pH 4.2 and after liming at various soil water potential were measured in centrifuged soil solution. Our results showed a significant gradual increase in the concentration of Al and most of mineral nutrients (Ca, Mg and P) with decreasing soil water potential from −3.5 kPa to −0.205 MPa. The results are important in the evaluation and interpretation of plant response to aluminium toxicity when accompanied by changes in water availability.